Chemical composition and antimicrobial activity of essential oil from Piper marginatum leaves obtained by hydrodistillation in pH4, pH7 and pH10

The present study aimed to evaluate possible variations in the chemical composition and antimicrobial activity of essential oils extracted from Piper marginatum leaves obtained by hydrodistillation at pH4, pH7, pH10. When compared, profiles three were different. A reduction major phenylpropanoids Z-asarone, dillapiole, E-asarone was observed for samples pH4-EO pH10-EO as compared sample pH7- EO. Sesquiterpenes macrocarpene (10.8%), viridiflorene (9.1%), caryophyllene (7.7%), E-nerolidol (6.5%) compounds identified sample. Compound ?-santonin (36.1%) a constituent oil pH4. also showed their minimum inhibitory concentration values (MIC). pH4 exhibited strong with an MIC 78.1 µg/ml both Escherichia coli Candida utilis microorganisms. We evidenced influence water pH on hydrodistillation, well its importance parameter studies using method.